Table 3.
Effects of auxins on rooting of in vitro elongated-shoos derived from leaf and nodal explants of H. enneaspermus after 5 weeks of culture
| Auxins concentration (mg/l) | Rooting frequency (%) | No. of roots/shoot | Length of the root (cm) | |||
|---|---|---|---|---|---|---|
| Leaf | Node | Leaf | Node | Leaf | Node | |
| Control | 0.0c | 0.0d | 0.0i | 0.0h | 0.0e | 0.0f |
| IBA | ||||||
| 0.5 | 78.4b | 70.4a | 6.2 ± 0.37f | 2.4 ± 0.24f | 5.30 ± 0.07b | 4.64 ± 0.05bc |
| 1.0 | 82.1a | 74.5a | 9.2 ± 0.37cd | 5.2 ± 0.48cd | 5.60 ± 0.08ab | 4.80 ± 0.18ab |
| 1.5 | 86.3a | 78.8a | 12.2 ± 0.37a | 8.8 ± 0.37a | 5.82 ± 0.03a | 5.10 ± 0.13a |
| 2.0 | 83.7a | 75.3a | 10.6 ± 0.24b | 6.2 ± 0.48bc | 5.70 ± 0.07a | 5.08 ± 0.08a |
| IAA | ||||||
| 0.5 | 74.4b | 64.5b | 4.2 ± 0.37g | 2.6 ± 0.24ef | 4.54 ± 0.08cd | 4.16 ± 0.30d |
| 1.0 | 79.8b | 70.7a | 8.4 ± 0.24de | 4.8 ± 0.37d | 4.52 ± 0.09cd | 4.18 ± 0.12d |
| 1.5 | 83.6a | 74.8a | 10.2 ± 0.37bc | 6.6 ± 0.40b | 4.72 ± 0.19c | 4.26 ± 0.06cd |
| 2.0 | 80.7a | 71.1a | 9.6 ± 0.40bc | 5.4 ± 0.24cd | 4.60 ± 0.11cd | 4.30 ± 0.20cd |
| NAA | ||||||
| 0.5 | 67.2 | 59.6c | 1.4 ± 0.24h | 1.2 ± 0.20g | 4.24 ± 0.14d | 3.50 ± 0.11e |
| 1.0 | 73.3b | 64.8b | 4.8 ± 0.37g | 2.6 ± 0.24ef | 4.32 ± 0.22d | 3.64 ± 0.09e |
| 1.5 | 77.6b | 68.2b | 7.8 ± 0.37e | 4.8 ± 0.37d | 4.48 ± 0.11cd | 3.74 ± 0.10e |
| 2.0 | 75.5b | 66.7b | 5.2 ± 0.20g | 3.6 ± 0.24e | 4.30 ± 0.08d | 3.60 ± 0.07e |
Control: MS basal medium. Values represent the mean ± standard error of three experiments. Mean values followed by the same letters within a column are not significantly different according to Duncan’s multiple range test at 5 % level