Table 4.

Effects of types and concentrations of polyamines on multiples shoot production in H. enneaspermus after 8 weeks of culture

PAs concentration (mg/l)	Multiple shoot regeneration frequency (%)		No. of multiple shoots/explant		Length of the shoot (cm)
	Leaf	Node	Leaf	Node	Leaf	Node
Control	88.4b	83.3a	34.80 ± 0.37d	29.40 ± 0.50d	5.60 ± 0.08b	5.44 ± 09b
Spermidine
5	81.3b	79.9b	28.80 ± 0.37f	24.60 ± 0.40f	6.56 ± 0.08a	6.30 ± 0.10a
10	85.7b	84.2a	32.00 ± 0.70e	26.60 ± 0.40e	6.60 ± 0.21a	6.46 ± 0.21a
15	90.5a	85.5a	37.20 ± 0.37b	31.40 ± 0.50bc	6.60 ± 0.07a	6.40 ± 0.10a
20	94.4a	89.6a	40.40 ± 0.50a	34.20 ± 0.48a	6.72 ± 0.06a	6.54 ± 0.09a
25	89.8b	83.7a	35.80 ± 0.37cd	30.80 ± 0.37c	6.52 ± 0.11a	6.42 ± 0.11a
Spermine
5	76.4c	71.1b	26.20 ± 0.48g	23.60 ± 0.40f	5.36 ± 0.17b	5.26 ± 0.06bc
10	80.6b	75.6b	29.40 ± 0.50f	26.20 ± 0.37e	5.50 ± 0.10b	5.34 ± 0.09bc
15	85.2b	81.5a	33.20 ± 0.48e	29.20 ± 0.48d	5.52 ± 0.08b	5.30 ± 0.18bc
20	90.1a	85.3a	36.60 ± 0.40bc	32.20 ± 0.37b	5.62 ± 0.13b	5.40 ± 0.11bc
25	84.7b	79.0b	32.20 ± 0.37e	28.60 ± 0.40d	5.54 ± 0.13b	5.30 ± 0.07bc
Putrescine
5	70.0c	66.5c	16.20 ± 0.37j	10.80 ± 0.37j	5.30 ± 0.09b	5.02 ± 0.13d
10	74.6c	69.4c	19.60 ± 0.24i	14.40 ± 0.24i	5.42 ± 0.11b	5.14 ± 0.10bc
15	78.8c	74.9b	24.60 ± 0.24h	18.60 ± 0.40h	5.40 ± 0.17b	5.12 ± 0.17bc
20	83.4b	77.5b	28.20 ± 0.37f	22.20 ± 0.48g	5.52 ± 0.21b	5.28 ± 0.09bc
25	86.5b	80.8a	32.20 ± 0.37e	26.40 ± 0.24e	5.52 ± 0.14b	5.36 ± 0.12bc

Control: MS medium supplemented with 4 mg/l BA + 1.5 mg/l NAA or 3 mg/l BA + 1.5 mg/l NAA for leaf and nodal explants respectively. Values represent the mean ± standard error of three experiments. Mean values followed by the same letters within a column are not significantly different according to Duncan’s multiple range test at 5 % level