Table 5.
Effects of polyamines on rooting of in vitro elongated-shoos derived from leaf and nodal explants of H. enneaspermus after 5 weeks of culture
| PAs concentration (mg/l) | Rooting frequency (%) | No. of roots/shoot | Length of the root (cm) | |||
|---|---|---|---|---|---|---|
| Leaf | Node | Leaf | Node | Leaf | Node | |
| Control | 86.4b | 78.6c | 12.60 ± 0.24c | 8.80 ± 0.20e | 5.56 ± 0.10b | 5.16 ± 0.19b |
| Spermidine | ||||||
| 5 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 10 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 15 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 20 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 25 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| Spermine | ||||||
| 5 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 10 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 15 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 20 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| 25 | 0.0c | 0.0d | 0.0f | 0.0g | 0.0c | 0.0c |
| Putrescine | ||||||
| 5 | 84.2b | 81.0b | 9.20 ± 0.20e | 7.60 ± 0.24f | 6.20 ± 0.23a | 5.74 ± 0.06a |
| 10 | 86.7b | 84.3b | 11.60 ± 0.40d | 9.20 ± 0.37e | 6.28 ± 0.09a | 5.68 ± 0.15a |
| 15 | 88.5b | 86.8b | 13.80 ± 0.37b | 10.80 ± 0.37c | 6.32 ± 0.08a | 5.82 ± 0.16a |
| 20 | 96.6a | 92.2a | 16.40 ± 0.24a | 12.00 ± 0.44a | 6.40 ± 0.31a | 5.90 ± 0.16a |
| 25 | 93.8a | 88.6b | 14.20 ± 0.37b | 11.40 ± 0.24ab | 6.32 ± 0.10a | 5.80 ± 0.04a |
Control: MS medium supplemented with 1.5 mg/l IBA. Values represent the mean ± standard error of three experiments. Mean values followed by the same letters within a column are not significantly different according to Duncan’s multiple range test at 5 % level