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. 2015 Jul 17;21(3):433–439. doi: 10.1007/s12298-015-0313-3

Table 2.

Effect of various BA/IBA and 2,4-D/TDZ combination on shoot regeneration parameters from leaf disc explants of Satureja avoromanica after 30 days of culture on MS and WPM media containing 0.7 % agar and 3 % commercial sugar

Concentrations (mgl−1) Frequency of response (%) Axillary Shoot number Main shoot length
Cytokinin Auxin MS WPM MS WPM MS WPM
0 0 15 10 1.00 ± 0.00G 1.33 ± 1.00E-G 0.98Q 0.98Q
BA IBA
 1 0.1 35 15 1.71 ± 0.37B-G 1.33 ± 0.2E-G 2.31 ± 1.2H-K 2.40 ± 1.2H-I
0.5 65 5 1.53 ± 0.88C-G 1.88 ± 0.00A-G 1.74 ± 1.2L-P 1.83 ± 0.00L-O
1 70 0 1.31 ± 0.42E-G 1.59 ± 1.2N-P
 2 0.1 90 25 2.00 ± 0.86A-G 1.80 ± 0.3B-G 2.33 ± 0.2H-K 2.54 ± 0.62GI
0.5 85 0 1.47 ± 0.75C-G 2.27 ± 0.56I-K
1 80 0 1.31 ± 0.82E-G 1.96 ± 0.45KN
 5 0.1 100 35 2.26 ± 1.2A-E 2.28 ± 1.00A-E 2.94 ± 0.30DF 3.09 ± 0.78CD
0.5 90 0 2.00 ± 1.2A-G 2.56 ± 0.50FI
1 90 0 1.58 ± 1.2C-G 2.00 ± 0.85K-M
 10 0.1 95 30 2.44 ± 0.6A-D 2.16 ± 1.10A-F 3.37 ± 0.40C 3.22 ± 0.90CD
0.5 90 0 1.94 ± 0.62A-G 2.46 ± 1.15HI
1 75 0 1.60 ± 0.15C-G 1.50 ± 0.45OP
TDZ 2,4-D
 1 0.1 15 25 1.66 ± 0.50B-G 1.80 ± 0.20B-G 1.63 ± 0.15M-P 3.02 ± 1.05CE
0.5 0 45 1.20 ± 0.25F-G 2.92 ± 0.65DG
1 0 50 1.45 ± 0.25D-G 2.67 ± 0.60EG
 2 0.1 20 90 2.25 ± 1.00A-E 2.66 ± 0.35AB 1.72 ± 0.17L-P 3.20 ± 0.35CD
0.5 0 80 1.72 ± 0.11B-G 2.86 ± 0.65DG
1 0 80 1.61 ± 0.15C-G 2.03 ± 0.55JL
 5 0.1 35 100 2.14 ± 0.75A-F 2.85 ± 0.56A 2.53 ± 0.29GI 4.28 ± 1.25A
0.5 0 85 2.23 ± 0.28A-E 2.97 ± 0.75DE
1 0 80 1.82 ± 0.13B-G 2.68 ± 1.2E-H
 10 0.1 30 95 2.50 ± 0.75A-C 2.50 ± 0.20A-C 3.88 ± 0.45B 4.34 ± 1.1A
0.5 0 80 1.18 ± 0.30F-G 2.68 ± 0.85GH
1 0 70 1.10 ± 0.15G 1.39 ± 0.70P

Means with the same letter for each parameter are not significantly different at the 5 % level according to DMRT