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. 2015 Aug 5;6:602. doi: 10.3389/fpls.2015.00602

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Copyright © 2015 Massange-Sanchez, Palmeros-Suarez, Martinez-Gallardo, Castrillon-Arbelaez, Avilés-Arnaut, Alatorre-Cobos, Tiessen and Délano-Frier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The overexpression (OE) of Ah24 in transgenic tobacco plants increases resistance to insect herbivory by leaf chewing larvae. Bars and error bars represent the mean values ± SE of (A) the weight gain in Manduca sexta larvae which fed on Ah24 OE transgenic plants having increasing transgene dosage [L2 (low), L15 (medium), and L14 (high)] or untransformed (WT) tobacco plants, and of (B) trypsin inhibitor activity (C) nicotine, and (D) jasmonic acid levels in unwounded leaves (UW; empty bars) and in damaged (local response, L; gray bars) and distal undamaged (systemic response, S; black bars) leaves of untransformed (WT) and transgenic Ah24 OE (L14) tobacco plants. All data was obtained using six plants per analysis (n = 6). Different letters over the bars represent statistically significant differences at P ≤ 0.05 (Tukey–Kramer test).