Table 1.
The Ah24 gene is induced in leaves of A. hypochondriacus plants subjected to insect herbivory (H), mechanical wounding (W), or methyl jasmonate (MeJA) treatments.
| Relative expressiona | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Hours post treatment (hpt) | 1 | 3 | 6 | 9 | 12 | 24 | 36 | ||
| Ah24 | H | Lb | – | 25 | 55 | 61 | 313 | 147 | 688 |
| Sb | – | 6 | 20 | 6 | 37 | 212 | 722 | ||
| W | L | 42 | 354 | 180 | 1064 | 1787 | 194 | 196 | |
| S | 1 | – | – | 32 | 23 | 4 | 3 | ||
| MeJA | L | 13 | 416 | 3782 | 3713 | 1091 | 2905 | 322 | |
| S | 3 | 3 | 12 | 33 | 85 | 125 | 77 | ||
| AhAOSc | H | L | – | 14 | 11 | 06 | 14 | 2 | 15 |
| S | – | 1 | 1 | 1 | 2 | 1 | 3 | ||
| W | L | 102 | 18 | 2 | 1 | 3 | – | 1 | |
| S | – | – | – | 1 | 2 | – | 1 | ||
| MeJA | L | 24 | 33 | 13 | 9 | 4 | 3 | 2 | |
| S | 3 | 3 | 6 | 14 | 6 | 6 | 9 | ||
| AhKTIc | H | L | – | – | – | – | 1 | 1 | 2 |
| S | – | 1 | 1 | 1 | 2 | 1 | 3 | ||
| W | L | 1 | 1 | – | 2 | 2 | 1 | 2 | |
| S | 1 | – | – | 7 | 5 | 2 | 3 | ||
| MeJA | L | 3 | 11 | 13 | 23 | 13 | 12 | 7 | |
| S | 5 | 7 | 17 | 63 | 27 | 19 | 43 | ||
a
The fold change in the expression of the target genes was calculated using the 2−ΔΔCt method according to Livak and Schmittgen (2001); controls were leaves from intact plants (for herbivory and mechanical wounding assays) or from plants treated with 0.01% Triton X-100 only (for MeJA assay).
b
L, local response; S, systemic response.
c
AhAOC, A. hypochondriacus Allene oxide cyclase; AhKIT, A. hypochondriacus Kunitz trypsin inhibitor.