Skip to main content
. 2015 Aug 5;35(31):11125–11132. doi: 10.1523/JNEUROSCI.4615-14.2015

Figure 1.

Figure 1.

Enhanced LTD in Eif4ebp2−/− mice is normalized by mGluR1 and mGluR5 antagonists. A, DHPG induced enhanced LTD in Eif4ebp2−/− mice compared with Eif4ebp2+/+ mice. B, The mGluR1 antagonist JNJ16259285 abrogated the difference in LTD between Eif4ebp2+/+ and Eif4ebp2−/− without affecting wild-type mice. This effect was prevented when anisomycin was coadministered. C, Fenobam, an mGluR5 antagonist, also normalized mGluR-LTD in Eif4ebp2−/− mice without affecting Eif4ebp2+/+ mice. Anisomycin cotreatment did not further affect the normalized mGluR-LTD. D, In the presence of anisomycin, mGluR-LTD was completely blocked in wild-type mice, but only normalized in 4E-BP2 mutant mice. E, fEPSP slope at 50–60 min after DHPG application for Eif4ebp2+/+ (n = 23), Eif4ebp2+/+ plus JNJ16259285 (n = 12), Eif4ebp2+/+ plus fenobam (n = 11), Eif4ebp2+/+ plus anisomycin (n = 12), Eif4ebp2−/− (n = 33), Eif4ebp2−/− plus JNJ16259285 (n = 15), Eif4ebp2−/− plus fenobam (n = 11), Eif4ebp2−/− plus anisomycin (n = 12), Eif4ebp2−/− plus JNJ16259285 and anisomycin (n = 10), and Eif4ebp2−/− plus fenobam and anisomycin (n = 8). Insets in A–D are representative traces. Calibration: 50 ms and 0.5 mV. **p < 0.01 vs Eif4ebp2+/+ plus vehicle; $p < 0.05 vs Eif4ebp2+/+ plus fenobam; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs Eif4ebp2−/− plus vehicle; &&&p < 0.001 vs Eif4ebp2−/− plus JNJ16259285; N.S. vs Eif4ebp2−/− plus fenobam.