Table 1.

Thermal cycling programs and primers used to generate DNA fingerprints using Rep-PCR.

Rep PCR	Primers	Thermal cycle
BOX	BOXA1R: 5′CTACGGCAAGGCGACGCTGACG	94°C 3 min 94°C 30 s × 35 52°C 30 s × 35 68°C 8 min × 35 68°C 15 min × 35 4°C hold
ERIC	ERIC1R: 5′ATGTAAGCTCCTGGGGATTCAC ERIC 2: 5′AAGTAAGTGACTGGGGTGAGCG	94°C 3 min 94°C 30 s × 35 52°C 30 s × 35 68°C 8 min × 35 68°C 15 min × 35 4°C hold
REP	REPR: 5′TTCGCYGGCAAGCCRGCTCC REP F: 5′GGCTTGCCRGCGAARRGGCC	94°C 3 min 94°C 30 s × 35 65°C 30 s × 35 72°C 8 min × 35 72°C 15 min × 35 4°C hold