Table 3.

Primers used for gene expression analysis by real-time PCR in posterior gut of European sea bass (Dicentrarchus labrax) fed cMOS (t = 60 days).

Gene	Name	Accession number	Forward (5′ → 3′)	Reverse (5′ → 3′)
IL-1β	Interleukin 1β	AJ269472	ATTACCCACCACCCACTGAC	TCTCTTCCACTATGCTCTCCAG-
IL-10	Interleukin 10	AM268529	ACCCCGTTCGCTTGCCA	CATCTGGTGACATCACTC
TNFα	Tumor necrosis factor α	DQ200910	ACAGCGGATATGGACGGTG	GCCAAGCAAACAGCAGGAC
IL-8	Interleukin 8	AM490063	GTCTGAGAAGCCTGGGAGTG	GCAATGGGAGTTAGCAGGAA
IL-6	Interleukin 6	AM490062	ACTTCCAAAACATGCCCTGA	CCGCTGGTCAGTCTAAGGAG
CD4	T-cell surface glycoprotein CD4	AM849811	GTGATAACGCTGAAGATCGAGCC	GAGGTGTGTCATCTTCCGTTG
CD8α	T-cell surface glycoprotein CD8 α chain	AJ846849	CTAAGATTCGGCAAAATAACTCGAC	GATGAGGAGTAGAAGAAGAAGGCC
MHCI	Major histocompatibility complex class I	AM943118	CAATACCTCACCCAGA	CTCCATCTTTCCTCCAGAT
MHCII	Major histocompatibility complex class II	AM113466	CAGAGACGGACAGGAAG	CAAGATCAGACCCAGGA
TCRβ	T-cell antigen receptor β chain	AJ493441	GACGGACGAAGCTGCCCA	TGGCAGCCTGTGTGATCTTCA
TGFβ	Transforming growth factor β	AM421619	GACCTGGGATGGAAGTGG	CAGCTGCTCCACCTTGTG
COX2	Ciclo-oxigenase 2	AJ630649	CATTCTTTGCCCAGCACTTCACC	AGCTTGCCATCCTTGAAGAGTC
IgL#18 (Ig)	Immunoglobulin	AJ400233	GAGCTGCAGGAGGACAGTG	TCAGACTGGCCTCACAGCT
Casp-3	Caspase 3	DQ345773	CTGATTTGGATCCAGGCATT	CGGTCGTAGTGTTCCTCCAT
Casp-9	Caspase 9	DQ345775	GGCAGGACTCGACGAGATAG	CTCGCTCTGAGGAGCAAACT
β-actin	β-actin	AJ537421	ATGTGGATCAGCAAGCAGG	AGAAATGTGTGGTGTGGTCG