Table 7.
Posterior gut relative gene expression profile of European sea bass (Dicentrarchus labrax) juveniles determined by RT-PCR after 60 days of dietary supplementation.
| Dietary treatment | Two-way ANOVA | ||||||
|---|---|---|---|---|---|---|---|
| FO | FOMOS | SBO | SBOMOS | MOS | OIL | M × O | |
| MHCII | 1.01 ± 0.12 | 1.14 ± 0.04 | 2.16 ± 0.43 | 1.88 ± 0.32 | NS | P < 0.05 | NS |
| MHCI | 1.03 ± 0.26 | 3.13 ± 0.15 | 0.77 ± 0.12 | 1.00 ± 0.26 | P < 0.05 | P < 0.05 | P < 0.05 |
| CD4 | 1.10 ± 0.17 | 2.02 ± 0.28 | 0.77 ± 0.09 | 1.07 ± 0.14 | P < 0.05 | NS | P < 0.05 |
| CD8α | 1.04 ± 0.25 | 1.62 ± 0.46 | 1.11 ± 0.33 | 1.49 ± 0.32 | P < 0.05 | P < 0.05 | NS |
| TCRβ | 1.04 ± 0.14 | 1.44 ± 0.14 | 0.82 ± 0.30 | 0.81 ± 0.30 | NS | P < 0.05 | NS |
| COX2 | 1.09 ± 0.16 | 1.76 ± 0.13 | 0.50 ± 0.09 | 0.66 ± 0.09 | P < 0.05 | P < 0.05 | P < 0.05 |
| IL-1β | 1.06 ± 0.16 | 1.02 ± 0.08 | 0.53 ± 0.13 | 0.83 ± 0.10 | NS | P < 0.05 | P < 0.05 |
| TNFα | 1.04 ± 0.12 | 1.98 ± 0.26 | 0.87 ± 0.13 | 1.27 ± 0.43 | P < 0.05 | P < 0.05 | NS |
| IL-8 | 1.08 ± 0.38 | 0.75 ± 0.11 | 0.71 ± 0.21 | 0.35 ± 0.20 | P < 0.05 | P < 0.05 | NS |
| IL-6 | 1.05 ± 0.22 | 0.57 ± 0.01 | 0.39 ± 0.05 | 0.48 ± 0.04 | P < 0.05 | P < 0.05 | P < 0.05 |
| TGFβ | 1.06 ± 0.17 | 0.26 ± 0.02 | 0.32 ± 0.02 | 0.60 ± 0.05 | P < 0.05 | P < 0.05 | P < 0.05 |
| IL-10 | 1.02 ± 0.13 | 1.06a ± 0.06 | 0.60 ± 0.09 | 0.75 ± 0.05 | NS | P < 0.05 | NS |
| IgL#18 | 1.06 ± 0.23 | 1.75 ± 0.03 | 0.48 ± 0.14 | 0.58 ± 0.23 | P < 0.05 | P < 0.05 | P < 0.05 |
Different letters within a line denote significant differences (P < 0.05) among dietary treatments (one-way ANOVA; Tukey’s test). Values expressed in mean ± SD of two pools of three specimens analyzed per tank (n = 3 tanks/diet).
NS, not significant.