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. 2015 Aug 5;3:47. doi: 10.3389/fcell.2015.00047

Figure 1.

Figure 1

Oncogenic Ras downregulates CD24 expression in NIH/3T3 cells. CD24 mRNA expression in vector control (Control) and RasV12 cells was determined by (A) RT-PCR and (B) RT-qPCR. RPLP0 was used as the loading and normalization control. CD24 mRNA levels shown as mean ± s.e.m. (C) Surface CD24 protein was determined by flow cytometry in Control and RasV12 cells. One representative histogram of isotype (Iso) and CD24-stained cells is shown. (D) Quantification of CD24 surface protein expression as mean ± s.e.m percentage of CD24+ cells. Significance was determined by Student's t-test, n = 3, **P < 0.01; ***P < 0.001.