Skip to main content
. 2015 Aug 5;3:47. doi: 10.3389/fcell.2015.00047

Figure 3.

Figure 3

The Raf and PI3K pathways downregulate CD24 mRNA expression while the Raf pathway is majorly responsible for downregulation of CD24 surface protein expression. (A) CD24 mRNA expression in Control, RasV12, RasV12G37, RasV12S35, and RasV12C40 cells was determined by RT-PCR. H-Ras mRNA expression was used to verify expression of ectopic Ras. RPLP0 was used as the loading control. (B) RT-qPCR was used to quantify CD24 mRNA expression with RPLP0 used as the normalization control. (C) Surface CD24 protein was determined by flow cytometry. One representative histogram of isotype (Iso) and CD24-stained cells is shown. (D) Quantification of CD24 surface protein expression as mean ± s.e.m percentage of CD24+ cells. Significance was determined by One-Way ANOVA with Tukey Honest Significant Difference analysis, n = 4, different lower case letters indicating different groups at P < 0.01.