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. 2015 Aug 5;3:47. doi: 10.3389/fcell.2015.00047

Figure 5.

Figure 5

Inhibition of Raf is sufficient to increase CD24 cell surface protein but not mRNA expression in RasV12 cells. (A–C) Rasv12 cells were treated for 16 h with DMSO (D) or 5, 10, and 20 μM sorafenib (S). (A) Western blot analysis was performed as in Figure 4. One representative experiment from three replicates is shown. CD24 mRNA expression in Control and RasV12 was determined by (B) RT-PCR and (C) RT-qPCR as in Figure 4. Significance was determined by One-Way ANOVA with Tukey Honest Significant Difference post-hoc analysis, n = 3. (D) Surface CD24 protein was determined by flow cytometry with Control and RasV12 treated for 24 h as above. One representative histogram of isotype (Iso) and CD24-stained cells is shown. (E) Quantification of CD24 surface protein expression as mean ± s.e.m percentage of CD24+ cells. Significance was determined by One-Way ANOVA with Tukey Honest Significant Difference analysis, n = 3, different lower case letters indicate different groups at P < 0.001.