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. 2015 Aug 5;3:47. doi: 10.3389/fcell.2015.00047

Figure 6.

Figure 6

Inhibition of PI3K did not alter the Raf-mediated inhibition of CD24 expression. (A–C) Rasv12 cells were treated for 16 h with DMSO (D), 100 μM LY294002 (LY) and/or 10 or 20 μM sorafenib (S/Sor) with LY. (A) Western blot analysis was performed as in Figure 4. One representative experiment from three replicates is shown. CD24 mRNA expression in Control and RasV12 was determined by (B) RT-PCR and (C) RT-qPCR as in Figure 4. Significance was determined by One-Way ANOVA with Tukey Honest Significant Difference post-hoc analysis, n = 3, a, bP < 0.01. (D) Surface CD24 protein was determined by flow cytometry with Control and RasV12 for 24 h as above. One representative histogram of isotype (Iso) and CD24-stained cells is shown. (E) Quantification of CD24 surface protein expression as mean ± s.e.m percentage of CD24+ cells. Significance was determined by One-Way ANOVA with Tukey Honest Significant Difference analysis, n = 3, different lower case letters indicating different groups at P < 0.001.