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Representative results of PCR amplification with primers P1 and P3 from genomic DNA isolated from different tissues of a Sod2lox/lox,SM-CreERT2(ki)Cre/+ mouse treated with OHT. Sense primer P1 and antisense primer P3 corresponding to sequences in Sod2 exons 2 and 3, respectively, delineate a 400-bp region in Sod2 alleles with floxed exon 3 excised. Lane M, 100-bp DNA ladder.
