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. 2015 May 6;309(3):C169–C178. doi: 10.1152/ajpcell.00046.2015

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Fig. 5. — Representative photomicrographs showing immunohistochemical staining of MnSOD in transverse sections of bladder specimens from wild-type and Sod2^lox/lox,SM-CreER^T2(ki)^Cre/+ mice, with or without OHT treatment, confirming that OHT treatment depleted MnSOD in detrusor SM of inducible SM-specific Sod2 deletion mice (modification: ×40). Bladder sections from a wild-type mouse without OHT injection (A), an OHT-treated wild-type mouse (B), and a Sod2^lox/lox,SM-CreER^T2(ki)^Cre/+ mouse without OHT injection (C) show positive staining of MnSOD in detrusor SM and urothelium. Conversely, a bladder section from an OHT-treated Sod2^lox/lox,SMCreER^T2(ki)^Cre/+ mouse (SM-specific Sod2^−/−) (D) shows positive staining of MnSOD in urothelium, but not in detrusor SM. U, urothelium.