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. 2015 May 29;309(3):H425–H433. doi: 10.1152/ajpheart.00438.2014

Fig. 5.

Fig. 5.

LSS reduces EMP release by SIRT1-mediated mitochondrial biogenesis. HUVECs were transiently transfected with either scrambled or SIRT1 small-interfering RNA (siRNA) and maintained in STT or LSS (20 dyne/cm2 for 24 h). A: SIRT1, PGC-1α, and TFAM protein expressions. α-Tubulin was used as a loading control (CTR). The bar graphs are results of densitometry analyses. B: mitochondrial content. C: activated- (CD62E+) or total- (CD31+/CD42a−) EMP. Each column represents means ± SE from 3–5 independent experiments. *P < 0.05; **P < 0.01. N.S., not significant.