Fig. 2.

During ENaC blockade, DIDS reduces total CO₂ absorption. J_tCO2 was measured during ENaC blockade (benzamil) in the presence and absence of a stilbene inhibitor (DIDS). Solid lines display results from experiments where the vehicle was applied in the first period and DIDS applied in the second period. Dashed lines indicate experiments where the order was reversed (i.e., DIDS was applied in the initial period and then removed in the second). Boxes show means ± SE of data under each condition. As shown, DIDS application changed tCO₂ flux from net absorption to net secretion.