Fig. 4.

Benzamil increases net H⁺ secretion. J_tCO2 and V_T were measured in the presence and absence of benzamil (A and C). This experiment was repeated during apical H⁺-ATPase blockade achieved with the application of bafilomycin to the perfusate (B and D). Solid lines display results of experiments in which vehicle was applied in the first period and benzamil was applied in the second. Dashed lines indicate experiments where the order was reversed (i.e., benzamil was applied in the initial period and then removed in the second). The boxes display means ± SE of all experiments performed.