Fig. 6.
During ENaC blockade, apical H+-ATPase inhibition increases Cl− absorption. JCl (A) and VT (B) were measured during ENaC blockade in CCDs from aldosterone-treated mice in the presence and absence of bafilomycin (5 nM) in the luminal fluid. Solid lines display results of experiments in which vehicle was applied in the first period and bafilomycin applied in the second. Dashed lines indicate experiments where the order was reversed (i.e., bafilomycin was applied in the initial period and then removed in the second). The boxes display means ± SE of all experiments performed.