Fig. 7.

Apical H⁺-ATPase inhibition reduces benzamil-sensitive V_T. V_T was measured before and after ENaC blockade in CCDs from aldosterone-treated mice in the presence (B) and in the absence (A) of bafilomycin (5 nM) in the luminal fluid. Solid lines display results of experiments in which vehicle was applied in the first period and benzamil was applied in the second. Dashed lines indicate experiments where the order was reversed (i.e., bafilomycin was applied in the initial period and then removed in the second). The boxes display the means ± SE of all experiments performed. C: benzamil-sensitive component of V_T measured in each group. *P < 0.05.