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H+-ATPase inhibition attenuates benzamil-induced Cl− secretion. The effect of benzamil on JCl and VT was examined in the presence and absence of apical H+-blockade achieved with bafilomycin (5 nM) added to the luminal fluid or with HCO3−/CO2 removed from the bath and perfusate. Benzamil-induced Cl− secretion (i.e., the benzamil-sensitive component of JCl) and benzamil-sensitive VT were calculated from data obtained under each of these treatment conditions. *P < 0.05, ANOVA.
