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. 2015 Jun 3;309(3):F204–F215. doi: 10.1152/ajprenal.00150.2015

Fig. 4.

Fig. 4.

Chronic HG does not increase mesangial cell proliferation. Cells were plated in NG or HG, and cell proliferation determined by counting cells (A), determining relative cell numbers with Alamar blue fluorescence (B), measuring incorporation of bromodeoxyuridine (BrdU) at the indicated time points by flowcytometry (C), and measuring the incorporation of BrdU at the indicated time points by ELISA after plating (D). Data are means ± SD; n = 3.