Fig. 2.

Repression of HKα promoter activity by phosphorylated and nonphosphorylated intracellular CagA is NF-κB-dependent. AGS cells were cotransfected with HKα206 or HKαΔ206 promoter-Luc reporter constructs and CagA or phosphorylation resistant (PR)-CagA expression vectors, and HKα promoter activity was measured by luminometry after 24 h to ensure maximal expression of the CagA vectors. Data are shown as means ± SD, n = 3. Inset shows CagA immunoblots of the corresponding lysates of CagA-transfected AGS cells, and β-actin served as a gel loading control.