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Repression of HKα promoter activity by the GM-3 analog Tri-DAP is NF-κB-dependent. AGS cells were transfected with HKα206 or HKαΔ206 promoter-Luc reporter constructs, treated with Tri-DAP or Tri-Lys, and HKα promoter activity was measured by luminometry. A: HKα206 promoter activity as a function of Tri-DAP concentration. B: HKα206 and HKαΔ206 promoter activities in the presence or absence of Tri-DAP or Tri-Lys. Data are shown as means ± SD, n = 3.
