Fig. 7.

Effects of HNG on hepatic TG secretion are independent of central insulin signaling. A and B: levels of GAPDH, total Akt, and phosphorylated (p)-Akt [Ser⁴⁷³ (S473)] in the hypothalamus by immunoblot (A) and quantification (B). C and D: levels of GAPDH, total Akt, and p-Akt (Ser⁴⁷³) in the liver by immunoblot (C) and quantification (D). E: serum TG levels in mice treated with SP (DMSO-SP; solid line with ⧫), HNG (DMSO-HNG; dashed line with □), and HNG + LY-294002 (LY-HNG; dashed line with △). F: serum TG levels in DMSO-SP (solid line with ⧫), DMSO-HNG (dashed line with □), SP + SHU9119 (SHU-SP; solid line with ●), and HNG + SHU-9119 (SHU-HNG; dashed line with △). *P < 0.05.