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. 2015 Jun 16;309(3):E302–E310. doi: 10.1152/ajpendo.00603.2014

Fig. 1.

Fig. 1.

Effect of (pro)renin receptor (PRR) siRNA on light chain 3B (LC3B) accumulation and autophagosome formation and protein levels of LC3BII, Lamp-2, and p62 in podocytes treated with high glucose. A: immunofluorescence staining of LC3B staining shown in green, 4,6-diamidino-2-phenylindole (DAPI) shown in blue (n = 4). B: summarized data show the fold change of relative LC3B fluorescence. C: Cyto-ID autophagosome staining shown in green, DAPI shown in blue (n = 4). D: summarized data show the fold change of relative autophagosome fluorescence. E: Western blot analysis of LC3B protein level in response to treatment with normal or high glucose for 48 h (n = 4 for each group). F: Western blot analysis of Lamp-2 protein level in response to treatment with normal or high glucose for 48 h (n = 4 each group). G: Western blot analysis of p62 protein level in response to treatment with normal or high glucose for 48 h (n = 4 for each group). NG, normal glucose, 5 mmol/l d-glucose; HG, high glucose, 25 mmol/l d-glucose; AP, autophagosome. Black bars, scrambled (Scr) siRNA; gray bars, PRR siRNA. Data are presented as means ± SE. *P < 0.05 vs. NG; #P < 0.05 vs. HG + Scr siRNA.