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. 2015 Aug 5;5:12628. doi: 10.1038/srep12628

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The bar chart shows the average activity measured in duplicate samples (± the s.e.m.). The lipid vesicles tested for each set of samples have the following lipid ratios: DOPE:DOPC 16:0 (columns 1 & 2), 12.8:2.8 (columns 3 & 4), 11.1:4.2 (columns 5 & 6), 8.0:7.0 (columns 7 & 8), 4.8:9.8 (columns 9 & 10) and 0:16 (columns 11 & 12) mol/mol, and then DOPC:DMPC 9.8:4.9 (columns 13 & 14), 7.0:8.1 (columns 15 & 16), 4.2:11.4 (columns 17 & 18) and 0:16 (columns 19 & 20) mol/mol. The proportion of PtdIns (4,5)P₂ substrate in the vesicles was constant at (DOPE + DOPC + DMPC): PtdIns (4,5)P₂/[³H]-PtdIns (4,5)P₂ 16:1 mol/mol. All other conditions are the same as for Fig. 2.