Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 22;6:7830. doi: 10.1038/ncomms8830

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Cartoon of CLASH-AChE/HCA. The acetylcholine esterase (AChE) inhibitor tacrine (T) displaces edrophonium (E) from AChE and benzenesulfonamide (SA) can bind to HCA inducing an increase in FRET efficiency. (b) Structure of the labelling compound. (c) Fluorescence micrographs of HEK293 cells displaying CLASH-AChE/HCA on their surface. In the absence of tacrine Cy3 emission is more intense, while on addition tacrine Cy5 emission increases; scale bars, 50 μm. (d) Changes observed in FRET ratio, donor (Cy3) and acceptor (Cy5) emission on perfusion of HEK293 cells displaying CLASH-AChE/HCA on their surface with 3 mM tacrine.