Figure 1. Josephin's aggregation and fibrillization process.

AFM morphology at (a) 0 day, (b) 2 days and (c) 7 days of incubation at 37 °C (scale bar, 1 μm). (d) Smaller observable fibrils with height in the order of 10–15 nm (7 days, scale bar, 0.5 μm). (e) Entangling fibrils with different growing heights (7 days, scale bar, 0.5 μm). (f) Supramolecular aggregate with height of ∼115 nm (7 days, scale bar, 0.5 μm). (g) Analysis of fibrillar morphology: fibrils' average convoluted width as a function of height. The width convolution effect was caused by the finite lateral dimensions of the tip, which contribute to the measured width of the structures. This unavoidable broadening effect is more important when imaging structures with dimensions smaller or comparable with the tip's apical radius (≈10 nm).