Figure 3. Comparison of gene sets involved in embryonic versus postembryonic development.

(a) The gene sets required for cuticle morphology (that is, epidermal patterning) during embryogenesis and typical insect metamorphosis are largely non-overlapping. This indicates that patterning principles may differ to quite some extent between these two stages of major morphological change. (b,c) This observation also holds true for the subsets affecting leg morphology (b) and GFP marked somatic muscles (c) indicating that both ectodermal and other patterning processes differ. (d) Gene sets required for ovary function. Many genes required for egg production in the pupal injection screen (green circle) were lethal in the larval injection screen. Hence, reduced egg production for these genes was probably due to starvation (green area outside hatched line). When comparing the non-lethal treatments (blue circle and green circle with hatched blue outline) the number of genes with an ovary phenotype in the pupal and larval injection screen are more similar. Insets: relations to the entire data set.