Figure 5. Postembryonic phenotypes.

(a) Wild-type ovary stained for F-actin (red) and DNA (blue). Pro-oocytes (asterisks) become encapsulated by somatic follicle cells and separated by stalk cells (arrow). (d) Upon Tc-MED24 RNAi, egg chambers are misarranged, not separated by stalk cells (arrow) and subsequently they fuse (IV). (b,c) After Tc-retained-RNAi the three most distal antennomeres (1–3) of the adult antenna are fused. (e,f) RNAi against Tc-ATP7 led to strongly reduced odoriferous gland content and partially melanized secretions (white arrowheads; remnant of posterior abdominal cuticle marked by open arrowhead:). (g–j) The knockdown of iB_04887 led to wing blisters in Tribolium pupae (arrowhead in h). Transgenic RNAi against the Drosophila ortholog showed the same phenotype, revealing a novel candidate for integrin mediated adhesion (arrowhead in j). Scale bars indicate 100 μm in (a–f) and 1 mm in (g–j).