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. 2015 Jul 24;6:7753. doi: 10.1038/ncomms8753

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Chromatin structure in the interphase nucleus of S. pombe expressing H2B-GFP observed using 3DSIM. Single optical sections are shown. Raw (wide field), deconvolution and 3DSIM images of a single nucleus of a live cell are shown, together with a single 3DSIM image of another fixed cell. The contrast of the images is adjusted to show the background with a very low noise level. Fixed cells showed chromatin structures similar to the live cells. Scale bar, 500 nm.