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. 2015 Jul 24;6:7753. doi: 10.1038/ncomms8753

Figure 4. Knobs are formed at ∼50 kb regions adjacent to the subtelomeric silent chromatin of chromosomes 1 and 2.

Figure 4

(a) A map describing the regions found adjacent to the telomeres of chromosomes 1 or 2. The silent subtelomere and ST-chromatin are ∼50 kb each. (b) Percentage of knob-containing nuclei in which the indicated lacO loci are proximal (<0.2 μm) to the knobs. Numbers in parenthesis indicate the number of nuclei examined. In the chromosome map, green regions are ‘ST-chromatin', orange regions are subtelomeric silent regions and purple regions are rDNA. (c) Two loci (sod2 and AC1) marked with a lacO array are found close to knobs, visualized with LacI-GFP (green). DAPI images are shown in blue in the merged image (left). The right panels show the DAPI images (white) superimposed with lacO/lacI-GFP and H3K9me3 regions (green and red contours, respectively) relative to the knob region (arrowhead). The positional relationships between the lacO/lacI-GFP, H3K9me3 and knob are drawn on the right. Scale bar, 500 nm. (d) Knobs in meiotic prophase nuclei. Telomeres are located on the left edge of the nucleus. Labels and drawings are the same as in c. Scale bar, 500 nm.