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. 2015 Jul 3;47(7):e171. doi: 10.1038/emm.2015.37

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Copyright © 2015 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Honokiol regulated the expression of PTX3 and inflammatory response in PA-induced HUVECs model. PTX-3 levels from the enzyme-linked immunosorbent assay (ELISA) (a), western blotting (b) and reverse-transcriptase PCR (RT-PCR) (c) in HUVECs after treatment with vehicle, 0.5 mM PA, PA plus 10 μM honokiol or PA plus an inhibitor of IKK-2 (TPCA-1, 30 μM) for 48 h. (d) Protein expression of IkB and p-IkB in HUVECs after treatment with vehicle, 0.5 mM PA or PA plus 10 μM honokiol for 48 h. (e) Protein expression of p50 and p65 in HUVECs after treatment with vehicle, 0.5 mM PA or PA plus 10 μM honokiol for 48 h. (f) IL-6, IL-8 and MCP-1 levels measured by the ELISA assay in HUVECs after treatment with vehicle, 0.5 mM PA or PA plus 10 μM honokiol for 48 h. Data are mean±s.d., n=3 in each group. *P<0.05, versus untreated group. ^#P<0.05 and ^##P<0.01, versus 0.5 mM PA treatment group.