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. 2015 Jul 10;47(7):e172. doi: 10.1038/emm.2015.39

Figure 1.

Figure 1

Overexpression of miR-103a-3p inhibits osteogenic differentiation and proliferation of hADSCs. (a) miR-103a-3p levels were determined in mimic control (mimic miR-cont) or mimic miR-103a-3p-transfected hADSCs using real-time PCR. (b) hADSCs proliferation was determined by direct cell counting after oligonucleotide transfection. (c) Oligonucleotide-transfected hADSCs were grown for 2 days and, when the oligonucleotide-transfected hADSCs were grown to 80–90% confluence, osteogenic differentiation was induced for 2 weeks and determined by Alizarin Red S solution, which was quantified by absorbance at 562 nm. (d) Real-time PCR analysis of ALP, in mimic miR-103a-3p-transfected undifferentiated and differentiated cells. Total RNA was isolated at days after induction of differentiation. Internal control for expression analysis was GUSB. Data represent mean±s.e.m. (n=4). *P<0.05 compared with mimic miR-cont-transfected hADSCs at 0 and 7 days. ALP, alkaline phosphatase; hADSC, human adipose tissue-derived stromal cell; miR, microRNA.