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. 2015 Jul 10;47(7):e172. doi: 10.1038/emm.2015.39

Figure 2.

Figure 2

Inhibition of miR-103a-3p increases osteogenic differentiation and proliferation of hADSCs. (a) miR-103a-3p levels were determined in inhibitor control-(IH-miR-cont) or IH-miR-103a-3p–transfected hADSCs using real-time PCR. (b) hADSCs proliferation was determined by direct cell counting after oligonucleotide transfection. (c) Oligonucleotide-transfected hADSCs were grown for 10 days and, when the oligonucleotide-transfected hADSCs were grown to 80–90% confluence, osteogenic differentiation was induced for 2 weeks and determined by Alizarin Red S solution staining, which was quantified by absorbance at 562 nm. (d) Real-time PCR analysis of ALP in IH-miR-103a-3p-transfeted undifferentiated and differentiated cells. Total RNA was isolated at days after induction of differentiation. Internal control for expression analysis was GUSB. Data represent mean±s.e.m. (n=4). *P<0.05 compared with IH-miR-cont transfected hADSCs at 0 and 7 days. ALP, alkaline phosphatase; hADSC, human adipose tissue-derived stromal cell; miR, microRNA.