Skip to main content
. 2015 Jul 10;47(7):e172. doi: 10.1038/emm.2015.39

Figure 4.

Figure 4

CDK6 siRNA inhibits osteogenic differentiation and proliferation of hADSCs. (a) CDK6 mRNA levels were determined in control-(si-cont) or CDK6 oligonucleotide (si-CDK6) transfected hADSCs by using real-time PCR. Internal control for expression analysis was GUSB. (b) hADSCs proliferation was determined by direct cell counting. (c) Oligonucleotide-transfected hADSCs were grown for 2 days and, when the oligonucleotide-transfected hADSCs were grown to 80–90% confluence, osteogenic differentiation was induced for 2 weeks and determined by Alizarin Red S solution staining, which was quantified by absorbance at 562 nm. (d) Real-time PCR analysis of ALP, and Runx2 in si-CDK6-transfected undifferentiated cells. Internal control for expression analysis was GUSB. Data represent mean±s.e.m. (n=4). *P<0.05 compared with si-cont-transfected hADSCs. ALP, alkaline phosphatase; hADSC, human adipose tissue-derived stromal cell; miR, microRNA; si-CDK6, CDK6 small interfering RNA.