FIG 3.
Srsf2 null fetal livers lack hematopoietic stem/progenitor cells. (A) Representative flow cytometry results from E14.5 FL cells. Lin-neg, lineage negative. Srsf2Δ/Δ FL lacked Lin-neg/c-Kit+ (LK) and Lin-neg/Sca1+/c-Kit+ (LSK) cells. (B) Quantification of the results presented in panel A. f/+, n = 10; f/f, n = 12; Δ/+, n = 5; Δ/Δ, n = 6. (C) Colony formation assay of E12.5 FL cells. Cells (2 × 104) were seeded in duplicate. f/+, n = 8; f/f, n = 24; Δ/+, n = 10; Δ/Δ, n = 6. Δ/Δ FL cells showed significantly lower colony-forming ability than others. (D) Representative results of Wright-Giemsa-stained cytospin processing of E14.5 PB. Δ/Δ PB lacked definitive red blood cells. (E) Quantification of the results presented in panel D. RBC, red blood cells. f/+, n = 4; f/f, n = 5; Δ/+, n = 8; Δ/Δ, n = 7. *, P < 0.05; **, P < 0.005.