FIG 5.

Overexpression of WT and overexpression of mutant SRSF2 result in similar phenotypes. (A) Protein expression of the overexpressed SRSF2 in mouse BM cells. β-Actin served as a loading control. Signal intensities of SRSF2 and the loading control were used to calculate the relative SRSF2 expression levels. (B) Growth curves of total BM cells in liquid culture. Means and SD of the results from three independent experiments are shown. All of the WT-, P95H-, and Δ8aa-expressing retrovirus-transduced cells showed a growth disadvantage compared to MIP control cells. (C) Apoptosis assay of cells on day 3. Combined data from independent three experiments are shown. P95H and Δ8aa cells showed enhanced apoptosis compared to MIP and WT cells. (D) Colony formation assay. Combined data from three independent experiments are shown. (E) BMT of SRSF2-overexpressing cells. Bone marrow cells were transduced with MigR1 vector, MigR1-SRSF2 WT, and MigR1-SRSF2 P95H retrovirus. Two days after the initial cell infection, cells were transplanted into lethally irradiated recipient mice. Five recipients were used in each group. Representative results of three independent experiments are shown. MigR1 recipients had a higher percentage of GFP in PB compared to their SRSF2 WT and P95H counterparts. *, P < 0.05; **, P < 0.005.