FIG 1.

Characterization of dnp300 mice. (A) dnp300 inhibits p300-dependent activation of the cyclin E promoter. The cyclin E-luciferase construct was cotransfected in HEK293 cells with E2F1 and p300 and with increasing amounts of the dnp300 construct. Luciferase activity was examined 16 h after transfections. (B) Typical picture of genotyping of dnp300 TR mice. TR, transgenic; W, wild type. (C) Expression of dnp300 transcripts in different tissues (shown at the top) was determined by QRT-PCR. H20, control without DNA; Pl, positive control using plasmid DNA. (D) Expression levels of endogenous WT p300 mRNA in tissues were examined by using QRT-PCR and normalized to values for the β-actin control. (E) Western blotting of the c-myc tag, endogenous p300, and the p300 homolog CBP. Bar graphs show the levels of the proteins as ratios to β-actin levels. (F) Blood parameters for dnp300 mice. Alterations of blood parameters are shown in red. BUN, blood urea nitrogen. (G) Levels of DGAT1 and DGAT2 proteins are reduced in livers of dnp300 mice. Western blotting was performed with Abs to DGAT1 and DGAT2. The filter was reprobed with β-actin. Bar graphs show levels of proteins as ratios to β-actin levels.