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. 2015 Aug 4;35(17):3005–3016. doi: 10.1128/MCB.00421-15

FIG 2.

FIG 2

Livers of dnp300 mice have alterations of chromatin structure and increased proliferation. (A, top) Immunofluorescence staining with anti-H3K9TriMe antibody and DAPI in livers of 2-month-old WT and dnp300 mice. (Bottom) Immunohistochemistry of livers with anti-H3K9TriMe antibody. The bar graph represents the percentage of hepatocytes with multiple positive foci. Data represent means ± SD (n = 3 to 5). *, P < 0.05. The right bottom section shows an examination of HP1α by Western blotting. Bar graphs show levels of HP1α as ratios to β-actin levels. (B) H&E staining of livers of 2-month-old WT and dnp300 mice. The bar graph represents the percentage of mitotic figures, which are denoted by arrows. Data represent means ± SD (n = 3). *, P < 0.05. (C) Examination of DNA replication by BrdU uptake and quantitation of the percentage of BrdU-positive hepatocytes. Bar graphs show a summary of data from three independent experiments with four mice of each genotype. Data represent means ± SD (n = 4). *, P < 0.05. (D and E) Protein levels of cyclin D1 and cyclin E (D) and of C/EBPα and C/EBPβ (E) were examined by Western blotting and densitometry calculations (bar graphs). Data represent means ± SD (n = 3 to 5). *, P < 0.05. CRM, cross-reactive molecules observed on cyclin E and C/EBPα filters. Bars, 20 μm.