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. 2015 Aug 4;35(17):3005–3016. doi: 10.1128/MCB.00421-15

FIG 4.

FIG 4

Livers of dnp300 mice have increased proliferation after partial hepatectomy. (A) BrdU uptake in the livers at different time points after PH. Black arrows show BrdU-positive hepatocytes; red arrows show mitotic figures. (B) Percentages of BrdU-positive hepatocytes at different time points after PH. (C) Examination of liver proliferation by Ki67 staining. Typical images of Ki67 staining are shown. Arrows show mitotic figures. (D) Numbers of Ki67-positive hepatocytes at each time point after PH. Bars for BrdU and Ki67 staining are 20 μm. (E) Percentages of mitotic figures at each time point after PH. (F) Expression of cell cycle proteins (shown on the left) after PH, determined by Western blotting. (G) Levels of PCNA protein calculated as ratios to the level of the β-actin control. Calculation of the ratios of cdc2, cyclin D1, and cyclin E levels to the β-actin level also confirmed the elevated levels of these proteins after PH in livers of dnp300 mice (data not shown). For panels B, D, E, and G, data represent means ± SD (n = 3 to 5). *, P < 0.05.