FIG 5.

Livers of dnp300 mice have decreased injury and increased proliferation after acute injection of CCl₄. (A) H&E staining of livers at different time points after CCl₄ injections. Bars, 20 μm. (B) Examination of serum ALT as a measure of liver injury at different time points after CCl₄ injections. (C) Immunohistochemistry of apoptotic cells in livers of WT and dnp300 mice was performed by using a TUNEL assay. Arrows show apoptotic hepatocytes. (D) Percentages of TUNEL-positive hepatocytes at each time point after CCl₄ injections. (E) Typical images showing BrdU uptake at different time points after injection of CCl₄. Arrows show BrdU-positive hepatocytes. (F) Calculation of percentages of BrdU-positive hepatocytes in livers of WT and dnp300 mice after injury. (G) Numbers of mitotic figures per 1,000 hepatocytes. For panels B, D, F, and G, data represent means ± SD (n = 4 or 5). *, P < 0.05.