TABLE 2.

Specific primers for each gene, annealing temperatures, and amplicon sizes

Gene	Direction	Primer	Annealing temp (°C)	Amplicon size (bp)
β-actin	Sense	5′-CCA GAG GCA TAC AGG GAC-3′	60	143
	Antisense	5′-TGA GGA GCA CCC TGT GCT-3′
Gapdh	Sense	5′-TCT TGC TCA GTG TCC TTG CTG G-3′	60	180
	Antisense	5′- TCC TGG TAT GAC AAT GAA TAC GC-3′
Pitx2c	Sense	5′-CCT CAC CCT TCT GTC ACC AT-3′	60	175
	Antisense	5′-GCC CAC ATC CTC ATT CTT TC-3
ccnd1	Sense	5′-TTG ACT GCC GAG AAG TTG TG-3′	60	154
	Antisense	5′-CTG GCA TTT TGG AGA GGA AG-3′
ccnd2	Sense	5′-ATG CTG CTC TTG ACG GAA CT-3′	60	201
	Antisense	5′-ATG CTG CTC TTG ACG GAA CT-3′
Pax7	Sense	5′-ACC ACT TGG CTA GGA TTT TCA AG-3′	60	240
	Antisense	5′- AGT AGG CTT GTC CCG TTT CC-3′
Myf5	Sense	5′-TGA GGG AAC AGG TGG AGA AC-3′	60	187
	Antisense	5′-AGC TGG ACA CGG AGC TTT TA-3′