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SUMOylation status of host affects SIF formation. Confocal microscopic analysis of HeLa cells with (YFP-SUMO1) or without (Untransfected) SUMO1 upregulation infected with different multiplicities of infection (MOIs) of mCherry-labeled S. Typhimurium for 7 h followed by immunostaining for LAMP1 to visualize the Salmonella-containing vacuoles (SCVs) and Salmonella-induced filaments (SIFs). Bar, 5 μm.
