FIG 2.

Musashi1 impact on splicing. (A) UCSC genome-browser image showing read coverage for RNA-Seq and iCLIP replicates in the gene ZAK. Region α highlights exons with no significant change in inclusion ratio, region β (short isoform) highlights exons with an increased inclusion ratio upon MSI1 knockdown, and region γ (long isoform) highlights exons with a decreased inclusion ratio upon Musashi1 knockdown. MSI1 knockdown shows a shift in usage between the two isoforms. (B) Schematic representation of panel A, showing the proportion of reads for ZAK falling within each of its exons in each replicate. (C) Relative mRNA levels for MSI1 after knockdown for qRT-PCR validation of ZAK splicing event show successful knockdown. (D) Relative mRNA levels as measured by qRT-PCR of the short ZAK isoform (containing region β), the long ZAK isoform (containing region γ), and a control upon knockdown of MSI1 confirm the same change in usage as observed in the RNA-Seq data.