Fig. 1.
Timeline of a model of reappearance of candidemia in sepsis [Candida+cecal ligation and puncture (CLP)]. In 24-h experiments, Candida albicans was intravenously injected at 6 h before cecal ligation and puncture (CLP) (−6 h), then subcutaneously administered antibiotic (ATB sc) was administered at 3 h and 8 h. Subsequently, the intravenous (tail vein) ascorbate (Asc) or normal saline (NSS) plus intraperitoneal amphotericin B 0.1 mg·kg−1·dose−1 (0.1 MK) or 1 mg·kg−1·dose−1 (1 MK) or intraperitoneal deoxyglycolate (De-cholate) were administered at 12 h and 18 h after CLP. The mice were euthanized at 24 h after CLP. In the 7-day survival experiment, additional ATBs were injected subcutaneously at 1, 2, 4, and 6 days after CLP, and intraperitoneal amphotericin B 1 mg·kg−1·dose−1 (Ampho B 1 MK) or De-cholate was given at 2, 4, and 6 days after CLP.