Figure 2.

Proline levels in salt- and PEG-treated rice cells. Suspension cultured cells of rice (cv Vialone nano) were treated with different, growth inhibitory concentrations of either NaCl (A,C,E) or PEG 6000 (B,D,F). At increasing time after the treatments, free proline levels (A,B) and total amino acid concentrations (C,D) were determined on a fresh weight basis. Proline concentration was also expressed as percent of total amino acids (E,F). All treatments were carried out in triplicate, and means ± SE are depicted. Very similar results were obtained also with cell cultures of the cv Loto.