Figure 6.

Expression of rice P5C dehydrogenase in E. coli and affinity purification. A truncated version of rice P5C dehydrogenase lacking the predicted mitochondrial transit peptide (Supplementary Figure 1) was expressed in E. coli, strain BL21 DE3 pLysS. Cells were analyzed by SDS-PAGE before and after the induction with 1 mM IPTG (A). Six hours after IPTG addition, cells were harvested and extracted, and the plant protein (⇨) was purified by affinity chromatography on a His-Select™ Nickel Gel column (B). The molecular mass of protein standards is indicated.